Fig. 2: Characterization of DNA origami-based aptamer nanoarray.
From: A DNA origami-based aptamer nanoarray for potent and reversible anticoagulation in hemodialysis
a Ethidium bromide-stained agarose gel image of the step-by-step assembly of DNA origami-aptamer nanoarray. Lane 1: Marker. Lane 2: M13 genome DNA (M13). Lane 3: rectangular DNA origami template with capture strands (Origami). Lane 4: DNA origami-TBA15 nanostructures (Ori-TBA15). Lane 5: DNA origami-HD22 nanostructures (Ori-HD22). Lane 6: DNA origami-aptamer nanoarray (Aptarray). b Hydrodynamic sizes and c Zeta potentials of DNA origami template (blue) and Aptarray (red). d AFM image of bivalent aptamer arrays on the DNA origami (36 TBA15, blue; 36 HD22, red; the four double rows of aptamers with distances of 5.4 nm between aptamer types). e Thrombin incubation with the Aptarray illustrated in panel d. The large areas of bright spots on the surface of the rectangular origami represent the bound thrombin molecules. Scale bars, 100 nm. f The heights of origami-based aptamer nanoarrays (blue, Aptarray; panel d) and thrombin bound structures (red, Aptarray + Thrombin; panel e) were measured by AFM. The gel image (a) and AFM graphs (d, e) are representative of three independent experiments. Source data are provided as a Source Data file.
