Fig. 3: T cell-specific knockout of DsbA-L alleviates diet-induced obesity and insulin resistance.

a Representative images of mice after a 12-week HFD feeding regimen starting at 8 weeks of age. b Body weight gain of DsbA-L^CD4-KO mice (n = 12) and control littermates (n = 7) during HFD feeding. c Body composition of DsbA-L^CD4-KO mice (n = 12) and control littermates (n = 7) fed an HFD for 12 weeks. d Representative images of fat pads and liver collected from DsbA-L^CD4-KO mice and control littermates fed an HFD for 12 weeks. e Relative tissue weights of HFD-fed DsbA-L^CD4-KO mice (n = 12) and control littermates (n = 7) fed an HFD for 12 weeks. f Representative H&E staining of eWAT, iWAT, BAT, and liver of DsbA-L^CD4-KO mice and control littermates fed an HFD for 12 weeks. Scale bars: 50 μm. g The average diameters of fat cells in DsbA-L^CD4-KO mice and control littermates were analyzed and quantified by the Image J software. h Fasting glucose levels in DsbA-L^CD4-KO mice (n = 4) and control littermates (n = 5) fed an HFD for 12 weeks. i Glucose tolerance test was performed in DsbA-L^CD4-KO mice (n = 12) and control littermates (n = 7) fed an HFD for 12 weeks. j Fasting insulin levels in DsbA-L^CD4-KO mice (n = 4) and control littermates (n = 5) fed an HFD for 12 weeks. k Insulin tolerance test was performed in DsbA-L^CD4-KO mice (n = 14) and control littermates (n = 11) fed an HFD for 12 weeks. Data shown are representative of three independent experiments. All data are presented as mean ± SEM. Statistical values p < 0.05 (*), p < 0.01 (**), p < 0.001 (***) are determined by two-tailed unpaired Student’s t-test. Source data are provided as a Source Data File.