Fig. 4: Comparison of TALE and dCas9 search dynamics in a heterochromatin locus.
From: TALEN outperforms Cas9 in editing heterochromatin target sites
a Multi-peak Gaussian fitting of normalized diffusion coefficient distribution reveals that the bimodal search behavior in heterochromatin is similar to that in euchromatin, but dCas9 kinetics is overall slower than TALE kinetics. NTALE 16 = 21647 and NCFTR Cas9 = 9424, where N denotes the number of data points. b Pointedly skewed angular distribution curve of gRNA9 demonstrates that its motion is highly constricted in heterochromatin. NTALE 16 = 1217 and NCFTR Cas9 = 22,800. Source data are provided as a Source Data file.
