Fig. 3: Identification of the wp mutation in the transcriptomes of B. dorsalis, C. capitata, and Z. cucurbitae.
From: White pupae phenotype of tephritids is caused by parallel mutations of a MFS transporter
The gray graphs show expression profiles from the candidate wp loci in WT (wp+) and mutant (wp−) flies at the immobile pupae stages of a B. dorsalis, b C. capitata, and c Z. cucurbitae. The gene structure (not drawn to scale) is indicated below as exons (arrows labeled E1–E4) and introns (dashed lines), the Major Facilitator Superfamily (MFS) domain is shown in blue. The positions of independent wp mutations (Bd: 37 bp deletion, Cc: approximate 8150 bp insertion, Zc: 13 bp deletion) are marked with black dashed boxes in the expression profiles and are shown in detail below the gene models based on de novo assembly of RNAseq data from WT and white pupae phenotype individuals (nucleotide and amino acid sequences). Deletions are shown as dashes, alterations on protein level leading to premature stop codons are depicted as asterisks highlighted in black. In situ hybridization on polytene chromosomes for d B. dorsalis, e C. capitata, and f Z. cucurbitae confirmed the presence of the wp locus on the right arm of chromosome 5 in all three species (arrows in micrographs). In situ hybridizations were done at least in duplicates and at least ten nuclei were analyzed per sample, scale bar = 10 µm. The source data underlying Fig. 3d–f are provided as a Source Data file.
