Fig. 1: Characterization of Met@Man-MPs.
From: Boosting anti-PD-1 therapy with metformin-loaded macrophage-derived microparticles
a Schematic of Met@Man-MPs as an efficient drug to boost anti-PD-1 therapy. Met@Man-MPs with MMP activity efficiently target to M2-like TAMs and degrade tumor collagen. (1) Met@Man-MPs repolarize M2-like TAMs to M1-like phenotype, resulting in the recruitment of CD8+ T cells into tumor tissues and the ameliorated tumor immunosuppressive microenvironment. (2) Collagen-degrading capacity of Man-MPs contributes to the infiltration of CD8+ T cells into tumor interiors and enhances tumor accumulation and penetration of anti-PD-1 antibody. (3) Met@Man-MPs synergistically inhibit tumor growth in combination with anti-PD-1 antibody, generating long-term memory immunity. b Hydrodynamic diameters of MPs and Man-MPs with or without Met by DLS analysis. Polydispersity index values are indicated in the brackets. c Zeta potentials of MPs and Man-MPs with or without Met by DLS analysis. Data are presented as means ± s.d. (n = 3 independent samples). d Morphology of MPs and Man-MPs with or without Met by TEM. Images are representative of three independent experiments. Scale bars: 500 nm. e Drug release of Met@MPs and Met@Man-MPs in PBS at different pH values. Data are presented as means ± s.d. (n = 3 independent samples). Source data are provided as a Source Data file.
