Fig. 5: rRNA variants are differentially expressed between tissues.

a Ribosome capture strategy for enriching mature ribosomes. Ribosomes are UV-crosslinked and captured with a magnetic bead coupled to a DNA/LNA mixamer with high affinity for the 25 S rRNA. b Graph depicting the relative quantification of 25S rRNA by qPCR after ribosome capture. Non-crosslinked ribosomes captured with the 25S LNA (25S), crosslinked ribosomes captured with the 25S probe (25S-XL), non-crosslinked ribosomes captured with an LNA probe that does not bind to the ribosome (Control), and crosslinked ribosomes captured with an LNA probe that does not bind to the ribosome (Control-XL). c Graph depicting the percentage of Illumina reads mapping to the ETS-ITS regions of the rDNA relative to the total amount of reads that map to the entire rDNA unit from total RNA and ribo-capture experiments. The reads from all tissues analyzed were used for this analysis. The ETS-ITS regions of the rDNA are present in the pre-rRNA but not present in mature ribosomes. d Heat map representing the allele frequency of SNPs found in the 25S of mature ribosomes in different tissue types. Blue boxes represent the highest value (8%), white boxes represent the lowest value (0%). The y-axis represents the positions of the SNPs with respect to the reference rDNA and are as follows: from top to bottom 4721, 4866, 4897, 4934, 5157, 5265, 5335, 5356, 5515, 5736, 5932, 6065, 6082, 6255, 6256, 6389, 6413, 6582, 6680, 6712, 6748, 6759, 6888, 7172, 7181, 7382, 7395, 7422, 7440, 7588, 7847, 7869, 7902, and 7987. The x-axis defines the different tissue types and sources: adult leaves (ALs), young leaves (YLs), inflorescences (INFLOs), siliques (S), online repository derived reads (DB), DNA (whole-genome sequencing data set).