Fig. 4: Hydrolytic activities of individual GH enzymes and their combinations with other enzymes encoded in the B. intestinalis EGE PUL.
From: Degradation of complex arabinoxylans by human colonic Bacteroidetes
a Hydrolytic traces of individual GH enzymes showing their end products on incubation with insoluble wheat arabinoxylan (InWAX). b–f Hydrolytic traces of multiple combinations of the enzymes encoded in the EGE PUL of B. intestinalis showing their end products on incubation with InWAX. g Hydrolytic traces of individual GH enzymes showing their end products on incubation with de-starched wheat bran (DWB). h–l Hydrolytic traces of multiple combinations of the enzymes encoded in the EGE PUL of B. intestinalis showing their end products on incubation with DWB. The end products of hydrolysis were identified and quantified by HPAEC-PAD for the three independent reactions reported in Figs. 3f, g, and one set of chromatograms is presented. Arabinose (A1), xylose (X1) and xylo-oligosaccharides (X2 to X6) were mixed and analyzed by HPAEC-PAD to serve as standards for the assignment of the released products.
