Table 1 Apparent affinities of DksA to wild-type and mutant RNAPs on the rrnBP1 promoter.

From: Structural basis of ribosomal RNA transcription regulation

RNAP

Kd,app (nM)

−ppGpp

+ppGpp

WT

2200 ± 430

860 ± 180

1

1

β′D256A

4540 ± 150

1640 ± 220

2.1

1.9

ΔσAL

3000

1150 ± 220

1.4

1.3

Δσ1.1

>10000

ND

WT RNAP + 

3900

2440 ± 1100

DksA D137A

1.8

2.8

  1. The apparent dissociation constants for DksA binding to promoter complexes (Kd,app) were calculated from the efficiency of transcription inhibition in titration experiments. The numbers in below Kd,app values indicate fold changes in Kd,app relative to the wild-type RNAP.
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