Fig. 5: Epistatic interactions of oc-1, boule, nanos-1, and eled. | Nature Communications

Fig. 5: Epistatic interactions of oc-1, boule, nanos-1, and eled.

From: Single-cell analysis of Schistosoma mansoni identifies a conserved genetic program controlling germline stem cell fate

Fig. 5: Epistatic interactions of oc-1, boule, nanos-1, and eled.The alternative text for this image may have been generated using AI.

a WISH images showing that the expression of oc-1 and boule are mutually dependent. Knockdown of either one causes the elimination of both. Asterisks: testes. b Confocal images showing representative individual testis lobules stained by DAPI and EdU in juvenile parasites after RNAi treatments. Dashed circles: testis lobule boundary. n: number of samples exhibiting the reported phenotype out of the total number of samples analyzed. c Fraction of EdU+ nuclei in testes in control and RNAi parasites. Each data point represents the average across all testis lobules in a single parasite. N = 10 (control RNAi); N = 10 (eled RNAi); N = 10 (oc-1 RNAi); N = 11 (eled;oc-1 RNAi); N = 10 (boule RNAi); N = 10 (eled;boule RNAi). nanos-1 RNAi experiments were not quantified because only a few GSC nuclei are present per testis lobule, which is insufficient for reliable statistics. Data are plotted as average ± standard deviation and p-values are calculated using two-sided Welch’s t-test. RNAi experiments were repeated on at least three biological replicates. d Proposed model of genetic interactions between oc-1, boule, nanos-1, and eled in regulating male GSC identity, proliferation, and differentiation.

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