Fig. 1: iCaspase9-mediated ablation of the germ cell lineage.
From: Direct allele introgression into pure chicken breeds using Sire Dam Surrogate (SDS) mating
a CRISPR/Cas9-mediated recombination of an iCaspase9 and GFP reporter gene into the final coding exon of the DAZL locus. Red arrow indicates the guide target. b 500 PGCs targeted with a human iCaspase9 or a chicken iCaspase9 (aviCaspase9) transgene were cultured in the presence of different concentrations of the B/B dimerisation compound for 10 days. Data are presented as the mean ± SD, n = 3 (n = 2 for 100 nM). One-way ANOVA, *P < 0.05, ***P < 0.001 with respect to Dazl-GFP cells. c Day-10 embryonic gonads were examined for expression of GFP and SSEA1 immunofluorescence, n = 4. d Control and (e) B/B-treated iCaspase9 G2 embryos imaged at day 10 of incubation. Arrows indicate the embryonic gonads. *, autofluorescence in the underlying mesonephros. n = 5 for each genotype. Scale bar, 100 μm.
