Table 1 Germline transmission rates from surrogate hosts injected with donor PMEL17 edited WL PGCs.

From: Direct allele introgression into pure chicken breeds using Sire Dam Surrogate (SDS) mating

iCaspase9 host mating groups

No. of eggs laid per week*

No. of eggs incubated

Fertilitya (% eggs incubated)

No. of chicks hatchedb (% fertile eggs)

cTransmission of host iCaspase9 transgene (%)

G01-15♂ x

G02-13♀

7.0

36

27 (75%)

17 (63%)

0

G02-22♀

G02-16♂ x

G02-13♀

5.78

66

51 (77%)

30 (59%)

0

G02-22♀

aviCaspase9 mating group

G02-08♂ G02-

09♂ x

4.43

82

68 (94%)

56 (82%)

9 (16%)

G01-19♀

G01-21♀G01-22♀

G1 generation mating groups

G124♂ x

5.13

43

29 (67%)

27 (93%)

0

G19♀ G112♀

G124♂ x

5.72

59

28 (47%)

25 (89%)

0

2 Line 6 WL ♀

  1. *Lay rate: eggs were counted over a 60 day period when hens were between 7 and 12 months of age and divided by the number of fertile hens present in pen. The maximum possible lay rate is 7.0 eggs per week.
  2. aFertility: no. of eggs with embryos at day 18 of incubation detected by candling.
  3. bHatchability: no. of chicks hatched from fertile day 18 eggs.
  4. cNo. of offspring PCR+ for iCaspase9 transgene.
Back to article page