Table 2 Germline transmission rates from surrogate hosts injected with donor KRT75-edited ♀LSX PGCs and wild-type ♂LSX PGCs.

From: Direct allele introgression into pure chicken breeds using Sire Dam Surrogate (SDS) mating

iCaspase9 host mating groups

No. of eggs laid per week*

Eggs incubated

Fertilitya (% of eggs incubated)

No. of chicks hatchedb (% fertile eggs)

cTransmission of host iCaspase9 transgene (%)

G04-16♂ x

G04-12♀

6.65

116

64 (55%)

43 (67%)

0

G04-19♀

G05-15♂ x

G05-5♀

6.48

27

16 (59%)

10 (63%)

0

G05-19♀

  1. *Eggs in pen were counted over a 60 day period when hens were between 10 and 13 months of age and divided by the number of fertile hens present in pen. The maximum possible lay rate is 7.0 eggs per week.
  2. aFertility: no. of eggs with embryos at day 18 of incubation detected by candling.
  3. bHatchability: no. of chicks hatched from fertile day 18 eggs.
  4. cNo. of offspring PCR+ for iCaspase9 transgene.
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