Fig. 4: RPA-binding is not required for the BTR complex to suppress SCEs, process UFBs, or promote DNA-end resection. | Nature Communications

Fig. 4: RPA-binding is not required for the BTR complex to suppress SCEs, process UFBs, or promote DNA-end resection.

From: The Bloom syndrome complex senses RPA-coated single-stranded DNA to restart stalled replication forks

Fig. 4

a RPA-binding by BLM and RMI1 is not required for suppression of SCEs (n = 3, one representative experiment shown). At least 10 metaphases were scored per sample. Error bars denote standard deviation (SD) from the mean. Significance was determined using the two-sided Mann–Whitney U test. b Airyscan high-resolution confocal microscopy images showing that both WT and RPA-binding (ΔRPA) mutant BLM proteins localize to UFBs (marked by RIF1, which localizes independently of BLM to UFBs68). Scale bars, 5 μm. c RPA-binding by BLM and RMI1 is not required for suppression of UFBs. Error bars denote standard error of the mean (SEM). Significance was determined by two-sided unpaired t test (n = 3). d RPA-binding by BLM and RMI1 is not required for DNA-end resection. Error bars denote SEM. Significance was determined by two-sided unpaired t test (n = 4).

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