Fig. 5: Stress-depleted intracellular SAM underlies nuclear leakage through lamin B1 maturation failure. | Nature Communications

Fig. 5: Stress-depleted intracellular SAM underlies nuclear leakage through lamin B1 maturation failure.

From: Cellular stress signaling activates type-I IFN response through FOXO3-regulated lamin posttranslational modification

Fig. 5: Stress-depleted intracellular SAM underlies nuclear leakage through lamin B1 maturation failure.

a Heatmap for metabolites extracted from NSPCs after 2 days of respective treatment. Data are presented as three independent experiments. SAM: S-adenosyl-methionine, SAH: S-adenosyl-homocysteine, DMG: dimethylglycine. b Diagram for lamin processing. c Left, IF images for mature lamin B1 (8D1, red) following 48 h of PQ or CL treatment. CL: 25 mM of cycloleucine (1-aminocyclopentanecarboxylic acid).  Right, quantitation for 8D1-positive nuclei. Scale bar = 10 µm. Mean ± s.e.m. of ten images. d WB results for total laminB1 (LMNB1) and mature laminB1 (8D1). IFNβ secretion in the media (e) and qRT-PCR analysis for ISGs (f) following 48 h of CL treatment. Mean ± s.e.m. of three independent experiments. g WB for defect of lamin processing and IFN-I activation in Icmt-targeted guide RNA transduced NSPCs (sg-Icmt). IFNβ secretion in the media (h) and qRT-PCR analysis for ISGs (i) following 48 h growth. Mean ± s.e.m. of three independent experiments. Statistical significance was determined by one-way ANOVA for (c) and by two-sided unpaired t-test for (e, f, h, and i). Experiments for c, d, and g were repeated three times independently with similar results and representative images/blots are shown.

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