Fig. 1: The absence of Emr1 leads to abnormal mitochondrial morphology.

a Maximum projection images of wild type (WT) and emr1Δ (emr1-deletion) cells expressing Cox4-GFP (a mitochondrial matrix protein) and mCherry-Atb2 (α-tubulin). Dashed lines mark the edge of cells and the red dashed line marks a cell lacking mitochondria. Note that mitochondria were tubular in WT cells but became spherical and/or aggregated in emr1Δ cells. Scale bar, 10 μm. b Quantification of mitochondrial phenotypes. On the left are representative images showing the indicated mitochondrial phenotypes. n indicates cell number observed for quantification. c Growth assays for WT and emr1Δ cells. The indicated cells were spotted on YE5S plates containing glucose (top) and YE5S plates containing glycerol (lower) after tenfold serial dilution to assess their growth on fermentable (glucose) and non-fermentable (glycerol) media, respectively. d Maximum projection time-lapse images of WT and emr1Δ cells expressing Cox4-GFP. Pink and green arrowheads mark mitochondrial fusion and fission, respectively. Scale bar, 10 μm. e Maximum projection time-lapse images of WT and emr1Δ cells expressing Cox4-GFP and mCherry-Atb2. Note that the cells were undergoing mitosis and one of the emr1Δ cells (bottom) segregated all mitochondria to only one end of the cell. Scale bar, 10 μm.