Table 1 IgH repertoire expressed by IgMneg B-1a cells in CKO mice contains class-switched IgH transcripts.

From: CTLA-4 expression by B-1a B cells is essential for immune tolerance

CTLA-4 B-cell CKO

B-1a subsets

% IgH isotype transcripts

IgM

IgD

IgG3

IgG1

IgG2b

IgG2c

IgE

IgA

#1

IgMhi

97.9

     

0.7

1

IgMneg

32.2

 

3.2

15.5

8.9

2.6

32.7

4.9

#2

IgMhi

99.9

       

IgMneg

33.2

 

31.1

4.9

17.3

8.6

1.7

3.1

#3

IgMhi

99.3

 

0.5

     

IgMneg

30.6

 

9.1

20.2

12.3

4.6

18.4

4.8

#4

IgMhi

99.3

 

0.1

     

IgMneg

65.4

4.1

4.4

6.4

2.7

1.4

4.8

10.8

#5

IgMhi

99.4

0.5

     

0.1

IgMneg

84.7

0.4

1

2

0.8

0.5

5.3

5.3

#6

IgMhi

98

0.5

    

0.7

 

IgMneg

6.4

0.1

2.2

5

0.9

0.9

15

69.5

#7

IgMhi

99.2

       

IgMneg

45.9

0.1

0.7

27.5

15.4

1.8

4.5

4.1

  1. IgMhi B-1a and IgMneg B-1a cells of CTLA-4 B-cell CKO mice were sorted and sequenced to obtain their IgH transcripts in each population. Percentage of IgH transcript expressing indicated Ig isotype for IgMhi B-1a and IgMneg B-1a cells from the indicated CKO mouse is shown in the column, n = 7 mice (2–3 months old).
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