Fig. 7: A plot of the rescaled histogram of the low-concentration image stack (blue bars) and the rescaled number (normalized) of clusters detected as a function of the binarization threshold (green dots). | Nature Communications

Fig. 7: A plot of the rescaled histogram of the low-concentration image stack (blue bars) and the rescaled number (normalized) of clusters detected as a function of the binarization threshold (green dots).

From: Nanoscale characterization of the biomolecular corona by cryo-electron microscopy, cryo-electron tomography, and image simulation

Fig. 7

The red dashed line shows the grayscale intensity bins that yield the largest number of clusters. Thresholds greater or less than these thresholds create artifacts in the solution owing to background noise. The accuracy in identifying the binarization threshold is highly dependent on the contrast of the images. The horizontal axis shows the grayscale intensity values. Both the histogram and the number of clusters have been normalized to their maximum value to permit comparison of peak locations.

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