Fig. 2: Polyamine transporter expression in pediatric DIPG brain tumors.
From: Dual targeting of polyamine synthesis and uptake in diffuse intrinsic pontine gliomas
Expression of polyamine transporter SLC3A2 in (a) DIPG tumors (n = 49) compared to normal fetal brain (n = 11) (p < 0.0001). Data were obtained from ZCC/PRISM Clinical trial and McGill University30. b–c Examination of RNA expression levels in a cohort of high-risk childhood cancers showed that the polyamine transporter, SLC3A2, was significantly overexpressed in DMG (n = 28) compared with high-risk childhood cancers (n = 148) (p < 0.0001) and neuroblastoma (n = 17) (p < 0.0001). Treatment of HSJD-DIPG007 cells with 40 mM DFMO leads to increased SLC3A2 (d) protein and (e) gene expression, resulting in (f) increased uptake of radiolabeled spermidine (p = 0.0276). g Polyamine transport inhibition by AMXT 1501 decreased uptake of radiolabeled spermidine and (h) was cytotoxic against patient-derived DIPG cell lines. Data are presented as mean values ± SEM of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. a, b, c, f Statistical analysis was calculated using two-tailed t tests between sample cohorts. e, g Statistical analysis was calculated using one-way ANOVA between cohorts and for treated and untreated samples. e UT vs 24 h: p = 0.0005, UT vs 48 h: p < 0.0001, UT vs 72 h: <0.0001, 12 h vs 24 h: p = 0.0107, 12 h vs 48 h: p = 0.0001, 12 h vs 72 h: p < 0.0001, 24 h vs 48 h: p = 0.0142, 24 h vs 72 h: p = 0.0021. g Control vs 0.01: p < 0.0001, Control vs 0.05: p < 0.0001, Control vs 0.01: p < 0.0001, 0.01 vs 0.05: p = 0.0092. 0.01 vs 0.1: p = 0.0026.
