Fig. 4: The effect of combined inhibition of polyamine synthesis and uptake, using DFMO and AMXT 1501, on cell proliferation, colony formation and apoptosis.
From: Dual targeting of polyamine synthesis and uptake in diffuse intrinsic pontine gliomas
HSJD-DIPG007 cells have synergistically decreased (a) cell survival and (b) form fewer colonies, upon combination treatment. c Synergy scores, calculated by Calcusyn, across a panel of neurosphere-forming DIPG cells treated with combination of both agents. d Annexin/PI staining of HSJD-DIPG007 cells treated with 40 mM DFMO, 5 µM AMXT 1501 or combination of both agents for 24 h. Vehicle vs DFMO: p = 0.0221, Vehicle vs AMXT 1501: p = 0.0003, Vehicle vs DFMO + AMXT 1501: p < 0.0001, DFMO vs DFMO + AMXT 1501: p < 0.0001, AMXT 1501 vs DFMO + AMXT 1501: p = 0.0005. e Apoptotic effects of DFMO and AMXT 1501 combination on protein expression of cleaved PARP and caspase 8. Representative blot from two independent experiments. Data are presented as mean values ± SEM of three independent experiments. a, b n = 3 wells examined over three independent experiments. d n = 3 independent experiments. **p < 0.01, ***p < 0.001. p-values were calculated using two-tailed t tests for treated and untreated cohorts.
