Fig. 6: Transcriptional events in late primary spermatocyte nuclei.
From: Dynamic sex chromosome expression in Drosophila male germ cells
a Labelled schematic of RNA Pol-II preinitiation, initiation, and elongation at a promoter. Optical sections of an immuno-stained L3 testis (b) and spermatocytes (c, d). Serine 2 phosphorylated Pol-II localization (green), the nuclear envelope marker Lamin (white), and the DNA stain DAPI (red). We indicate spermatogonia and spermatocytes, separated by a line (dashed, white). Three/five visible pigment cells are indicated. c Serine 2 phosphorylated Pol-II localization (green) and total Pol-II (red). d Serine 5 phosphorylated Pol-II localization (green) with total Pol-II (red). c, d From left to right: DAPI grayscale, Phospho-CTD grayscale, total Pol-II grayscale, color merge. Primary spermatocyte nuclei are outlined (white-dashed line) based on DAPI. Insets in the merged images are anti-Asterless staining (grayscale) used to stage by centriole length, a measure of spermatocyte age. The ratio of e Serine 2 phosphorylated Pol-II (261 territories scored) or f Serine 5 phosphorylated Pol-II to total Pol-II pixel intensity on the X chromosome and autosome territories (N = 148 territories). We corrected for background using non-territory nuclear staining in the same nucleus. Significance (p ≤ 0.01, two-sided Welch’s t-test) (asterisk).
