Fig. 5: Compound 5h combined with remdesivir does not permit viral breakthrough.
VeroE6 cells were exposed with SARS-CoV-2WK-521 for 1 h, the virus was washed out, and the virus-exposed cells were cultured for 3 days in the presence of compounds. a Viral RNA copy numbers in the culture supernatants were determined using RNA-qPCR. The numbers of viral RNA copy were more significantly reduced in the combination of 5h and remdesivir (each at 4, 10, 20, and 40 µM) than 5h or remdesivir alone. The mean EC50 values of 5h and remdesivir determined with this set of the data were 4.0 and 9.0 µM, respectively. The figure was generated with GraphPad Prism 9. Bars indicate geometric mean (n = 2). b Representative images of fluorescent immunocytochemistry data from two independently conducted experiments are shown. SARS-CoV-2 antigens, F-actin, and nuclei are indicated in green, red, and blue, respectively. Source data are provided as a Source Data file.
