Fig. 3: Divergent SCOOP peptides induce MIK2-dependent responses.
From: Perception of a divergent family of phytocytokines by the Arabidopsis receptor kinase MIK2
a Alignment of SCOOP motifs from A. thaliana. b ROS production in leaf disks collected from 4-week-old A. thaliana plants induced by application of 1 μM SCOOP peptides in Col-0 (black) or mik2-1 (green) (n = 8 leaf disks). A line represents mean; error bars represent S.D. P-values indicate significance relative to the WT control as indicated by two-tailed T test c Fresh weight of 14-day-old seedlings grown in the presence of 1 μM SCOOP peptides for 10 days relative to mock. A line represents mean; error bars represent S.D. (n = 12 seedlings). A two-way ANOVA indicated a significant effect of treatment and genotype (P < 0.0001); P-values indicate significance relative to Col-0 in a Sidak’s multiple comparison test between genotypes within each treatment. d Co-immunoprecipitation of BAK1 with MIK2–GFP from mik2-1/MIK2-GFP seedlings treated with 1 μM SCOOP peptides, or water for 10 min. Western blots were probed with antibodies α-GFP and α-BAK1. These experiments were performed and analyzed three times with similar results. These experiments were performed and analyzed three times with similar results. ROS reactive oxygen species.
