Fig. 3: Potentiation of eVD metabolism by AEA.

a Metabolism 10 µM NADA and b 10 µM NA5HT in the presence of increasing concentrations of AEA by BV2 microglia. Metabolism was conducted similarly to data in Fig. 2c. Data shown are the mean ± SEM of n = 6 (two sets of triplicate performed on separate days). Statistical significance is based on a one-way ANOVA with a Tukey’s post-hoc. c Schematic of the CYP2J2-CPR-Nanodisc. d, e Metabolism of NADA and NA5HT by CYP2J2-CPR-NDs. Rates are in pmol_epoxy-eVD min⁻¹ nmol_CYP2J2⁻¹ and data represents the mean ± SEM of three independent experiments. f–i Co-substrate metabolism of eVDs with AEA. f AEA metabolism in the presence of NADA and g NA5HT. Rates are shown as the fraction of the V_max (135 pmol_EET-EAs min⁻¹ nmol_CYP2J2⁻¹) for AEA metabolism (gray squares) as previously published (see text). h Fold change of NA5HT metabolism and i NADA metabolism in the presence of increasing AEA concentrations compared to the metabolism without AEA. f–i Data represents the mean ± SEM of three independent experiments. All data can be found in the Source Data file.