Fig. 5: AgPd0.38 as coating additive enabled an inert surface to inhibit biofilm formation in vitro.
From: Surface-bound reactive oxygen species generating nanozymes for selective antibacterial action
a, b Schematic illustration on the preparation of AgPd0.38-coated PDMS surface (i.e., PDMS/PDA/AgPd), by coating a circular PDMS wafer successively with dopamine and AgPd0.38. c (1st row) SEM images of (from left to right) the pristine PDMS surface, the intermediate PDMS/PDA surface, and the final PDMS/PDA/AgPd surface. (Inset) Zoomed-out SEM image of PDMS/PDA/AgPd, with a white arrow indicating an AgPd0.38 particle. (2nd row) SEM images of (from left to right) PDMS, PDMS/PDA, and PDMS/PDA/AgPd surfaces after a 5-day incubation with P. aeruginosa PAO1 in nutrient broth. Ellipses outlined by white dashed lines indicate intact P. aeruginosa PAO1 cells. P. aeruginosa PAO1 is a Gram-negative bacterium that is naturally green fluorescent and has a strong tendency to form biofilms. (3rd row) Reconstructed 3-dimensional confocal fluorescence microscopy images of P. aeruginosa PAO1 cells on (from left to right) PDMS, PDMS/PDA, and PDMS/PDA/AgPd after 5-day co-incubation in nutrient broth.
