Fig. 2: Functional identification of flavone reductase (FLR).
From: Discovery of an ene-reductase for initiating flavone and flavonol catabolism in gut bacteria
a The reaction system, coupling NADH consumption and FMNH2 generation, for the assay of FLR activity towards apigenin. Fre, NADH-specific FMN oxidoreductase. b HPLC analysis of the products formed by the in vitro FLR-catalyzed conversion between apigenin (Api) and naringenin (Nar). The control reactions were carried out using the disabled FLR (boiled). c In vitro steady-state kinetic analysis of FLR using apigenin or naringenin as the substrate. Each data point represents mean ± SD (n = 3). Error bars show SDs. d A screening of the substrate spectrum of FLR. Initial turnover rates of purified FLR towards different substrates (100 μM) were measured. Data are represented as mean ± SD (n = 3). Error bars show SDs.
