Table 1 Contacts of Get5 to ribosomal proteins identified by mass spectrometry.

From: Ribosome-bound Get4/5 facilitates the capture of tail-anchored proteins by Sgt2 in yeast

Hit

MW (kDa)

Coverage (%)

p value

Total spectra

His6-Get5/Get5

(total spectra)

Rpl20a (eL20)

20.4

62

0.0001

405

1.45

Rpl23a (uL14)

14.5

43

0.0078

31

1.47

Rpl26b (uL24)

14.2

30

0.00036

23

1.44

Rpl37a (eL37)

9.9

25

0.0088

25

1.60

Rps19a (eS19)

15.9

34

0.00011

21

1.47

Rps29a/b (uS14)

6.7

70

0.0044

71

1.79

His6-Get5

23.7

84

0.0001

757

5.29

Get4

36.3

43

0.0001

152

3.15

Sgt2

37.2

14

0.0015

9

n.d. (0 in Get5 control)

  1. Given are the conventional yeast names of ribosomal proteins and in brackets their names according to the unified nomenclature77. The p value was determined by a one-way ANOVA test (performed in Scaffold and followed by a Benjamini–Hochberg correction) and core ribosomal proteins with a p value <0.01 were selected according to their enrichment in the His-tagged sample with a 1.4-fold enrichment used as a threshold (see Supplementary Dataset 1).
Back to article page