Fig. 1: Plasmonic cultivation surface activated by a laser of appropriate wavelength induces microthermal damage. | Nature Communications

Fig. 1: Plasmonic cultivation surface activated by a laser of appropriate wavelength induces microthermal damage.

From: Microthermal-induced subcellular-targeted protein damage in cells on plasmonic nanosilver-modified surfaces evokes a two-phase HSP-p97/VCP response

Fig. 1

a Schematic representation of the concept of microthermal damage inflicted on cellular proteins. The cell culture plate surface is modified by a thin polymeric film consisting of PAA (polyacrylic acid) and PDDA (poly(diallyl dimethylammonium chloride) for the efficient binding of plasmonic silver NPs. Plasmon NPs convert energy from light (laser) immediately and efficiently to heat, enabling direct focusing of the heat on subcellular regions. b. Thermal imaging shows emitted heat detected in the LWIR spectrum in plasmon-modified cell culture plate wells activated by 561 nm laser. c Recruitment of various GFP-tagged heat shock-related proteins to micro-heated regions in U-2-OS cells grown on a plasmon-modified Ibidi plate. Microheated regions were exposed to 561 nm laser (power 15%). The defined laser path is shown in white. Cells were followed in time. Representative results from two experiments. Scale bars = 10 µm, for TEM micrograph 100 nm.

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