Fig. 3: Impaired recruitment of several HSP70 mutants and characterization of proteins damaged by micro-heating. | Nature Communications

Fig. 3: Impaired recruitment of several HSP70 mutants and characterization of proteins damaged by micro-heating.

From: Microthermal-induced subcellular-targeted protein damage in cells on plasmonic nanosilver-modified surfaces evokes a two-phase HSP-p97/VCP response

Fig. 3

a U-2-OS cells grown on a plasmon-modified Ibidi plate expressing HSP70-GFP variants were micro-heated by laser stripes and followed in time, revealing impaired recruitment to micro-heated regions of the indicated HSP70 mutants, compared to wild-type (WT) HSP70 control. b U-2-OS CHIP-GFP cells were micro-heated in the form of colinear stripes, fixed and processed for immunofluorescence analysis of the accumulation of K48- and K63-ubiquitinated proteins. c Detection of amyloid aggregates by NIAD-4 and AmyloGlo dyes in U-2-OS cells in microheated regions. The defined laser path is shown in the upper left corner of each image. All panels show representative results from two experiments. Scale bars =  10 µm.

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