Fig. 5: The effect of HSP70 inhibition on recruitment of HSP70, ubiquitin and p97 translocase. | Nature Communications

Fig. 5: The effect of HSP70 inhibition on recruitment of HSP70, ubiquitin and p97 translocase.

From: Microthermal-induced subcellular-targeted protein damage in cells on plasmonic nanosilver-modified surfaces evokes a two-phase HSP-p97/VCP response

Fig. 5

a Pretreatment by HSP70 inhibitor (VER155008, 20 µM for 30 min) increases the persistence of HSP70-GFP in micro-heated regions. b Inhibition of HSP70 by VER155008 (20 µM for 30 min) promotes ubiquitination of heat-damaged proteins detected by GFP-ubiquitin reporter. c Inhibition of HSP70 by VER155008 (20 µM for 30 min) increases the recruitment of p97-GFP to micro-heated regions. All panels show representative results from three experiments.

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