Table 3 Cryo-EM data collection, refinement and validation statistics.
Pangolin-CoV (EMD-12130) (PDB 7BBH) | |
|---|---|
Data collection and processing | |
Voltage (kV) | 300 |
Electron exposure (e–/Å2) | 51.8 |
Defocus range (μm) | −1.5 to −3.0 |
Pixel size (Å) | 1.08 |
Symmetry imposed | C3 |
Final particle images (no.) | 93 k |
Map resolution (Å) | 2.9 |
FSC threshold = 0.143 | |
Map resolution range (Å) | 2.8–3.6 |
Refinement | |
Initial model used (PDB code) | 6ZGE |
Model resolution (Å) | 3.0 |
FSC threshold = 0.5 | |
Map sharpening B factor (Å2) | −86.5 |
Model composition | |
Non-hydrogen atoms | 25,827 |
Protein residues | 3189 |
Ligands | 69 |
B factors (Å2) | |
Protein | 38.0 |
Ligand | 70.9 |
R.m.s. deviations | |
Bond lengths (Å) | 0.005 |
Bond angles (°) | 0.707 |
Validation | |
MolProbity score | 1.36 |
Clashscore | 3.02 |
Poor rotamers (%) | 0.86 |
Ramachandran plot | |
Favored (%) | 96.13 |
Allowed (%) | 3.87 |
Disallowed (%) | 0.00 |
