Fig. 8: Histone H3.3 somatic alterations in pediatric brain tumors include H3F3C mutations.

a Prevalence and distribution of somatic SNVs or indels within H3F3A (left, involving 36 tumors and 32 patients) and H3F3C (right, involving nine tumors and nine patients), based on CBTCC tumors. b Across the 143 impacted CBTTC tumors, somatic mutations involving H3F3A, H3F3C, and TP53, as well as TP53 copy loss. c For CBTTC cohort, associations of somatic alterations involving H3F3A, H3F3C, or TP53 with overall burden of SSVs. Numbers of tumors in each group from c, with 711 tumors in “unaligned” group. P values by linear model correcting for tumor type. d Prevalence and distribution of somatic SNVs or indels within H3F3C in TCGA adult pan-cancer cohort involving 49 patients (out of 10224 with whole-exome sequencing). e For TCGA cohort, associations of somatic alterations involving H3F3A, H3F3C, POLE⁵⁶, or TP53 with the total number of detected exome SNV/indel mutations. P values by linear model correcting for tumor type. See Methods regarding tumor type abbreviations. Box plots represent 5% (lower whisker), 25% (lower box), 50% (median), 75% (upper box), and 95% (upper whisker).