Fig. 1: Rapid labeling of cell-surface iGluRs by ligand-directed two-step labeling.
From: Ligand-directed two-step labeling to quantify neuronal glutamate receptor trafficking
a Schematic illustration of the two-step labeling to iGluRs. In the 1st step, a strained alkene is covalently attached to iGluRs by LDAI chemistry. In the 2nd step, Tz probe is selectively tethered by IEDDA reaction. Lg, selective ligand for iGluRs; Nu, nucleophilic amino acid residue. Glu, glutamate. b Chemical structure of CAM2(TCO). c Chemical structure of Tz probes. The detailed chemical structures are shown in Supplementary Fig. 2. d Schematic illustration of the two-step labeling in live cell.
