Fig. 2: miR-33−/− mice exhibit reduced oxygen consumption in a cold environment.
From: microRNA-33 maintains adaptive thermogenesis via enhanced sympathetic nerve activity
a Oxygen consumption rate in miR-33+/+ and miR-33−/− mice kept at 23 °C. n = 12 mice per group, **p < 0.01, fdANOVA. b Carbon dioxide production rate in miR-33+/+ and miR-33−/− mice kept at 23 °C. n = 12 mice per group, *p < 0.05, fdANOVA. c Mean oxygen consumption rate during the light and dark phase in miR-33+/+ and miR-33−/− mice kept at 23 °C. n = 12 mice per group, *p < 0.05, two-sided unpaired t test. d Mean carbon dioxide production rate in miR-33+/+ and miR-33−/− mice kept at 23 °C. n = 12 mice per group. ***p < 0.001, two-sided unpaired t test. e Respiratory exchange rate in miR-33+/+ and miR-33−/− mice kept at 23 °C. n = 12 mice per group. f Locomotor activity of miR-33+/+ and miR-33−/− mice kept at 23 °C. n = 10 mice per group. g Oxygen consumption rate in miR-33+/+ and miR-33−/− mice at 2 days after transfer from 23 to 18 °C. n = 10 mice per group, p < 0.05, fdANOVA. h Carbon dioxide production rate in miR-33+/+ and miR-33−/− mice at 2 days after transferring from 23 to 18 °C. n = 10 mice per group, p < 0.05, fdANOVA. i Mean oxygen consumption rate in miR-33+/+ and miR-33−/− mice at 2 days after transferring from 23 to 18 °C. n = 10 mice per group, *p < 0.05, two-sided unpaired t test. j Mean carbon dioxide production rate in miR-33+/+ and miR-33−/− mice at 2 days after transferring from 23 to 18 °C. n = 10 mice per group. **p < 0.01, two-sided Mann–Whitney test. k Respiratory exchange rate in miR-33+/+ and miR-33−/− mice at 2 days after transferring from 23 to 18 °C. n = 10 mice per group. *p < 0.05, two-sided unpaired t test. l Locomotor activity of miR-33+/+ and miR-33−/− mice at 2 days after transferring from 23 to 18 °C. n = 9 mice per group. m Maximum oxygen consumption after 1.0 mg/kg subcutaneous norepinephrine (NE) injection. miR-33+/+ and miR-33−/− mice at 3 days after transfer from 23 to 18 °C were used. Measurements were performed at 30 °C chambers. n = 15, 13 mice per group, *p < 0.05, two-sided Mann–Whitney test for each time. n Serial measurement of BAT and rectal temperature in miR-33+/+ and miR-33−/− mice at a cold condition. n = 13, 11 mice per group. *p < 0.05, two-sided Mann–Whitney test. All data are presented as mean ± SEM.
