Table 2 Sequences of circular DNA, primers, and probes used in this study.
From: Rapid electrochemical detection of coronavirus SARS-CoV-2
Type | Name | Sequence (5′ → 3′) | Length (bases) |
|---|---|---|---|
Universal capture probe | CP | CGCAACTGAACTACTTGTCG–biotin | 20 |
Blocking probe | BP | Biotin-TTTTTTTTTT | 10 |
For N gene | |||
Forward primer | Ng_SARS2_F | TCATCACGTAGTCGCAACAG | 20 |
Reverse primer | Ng_SARS2_R | CAAAGCAAGAGCAGCATCAC | 20 |
Circular DNA | RCA-Ng | TACGTGATGACGCAACTGAACTACTTGTCGCTGTAGTTCAAGATATCGCGTCCTACCTGTTGCGAC | 66 |
Reporter probe | Ng-RP | CAAGATATCGCGTCCTAC–biotin | 18 |
Linear target | Ng-LT | GTTCCTCATCACGTAGTCGCAACA GTTCAA | 30 |
Mismatched target | Ng-MM | GTTCCTCATCACGTTCTCGCAACA GTTCAA | 30 |
For S gene | |||
Forward primer | Sg_SARS2_F | TACCCATTGGTGCAGGTATATG | 22 |
Reverse primer | Sg_SARS2_R | AGTGTAGGCAATGATGGATTGA | 22 |
Circular DNA | RCA-Sg | ACCAATGGGTCGCAACTGAACTACTTGTCGCTGTAGTTATTCTGTCATGCGCTCACATATACCTGC | 66 |
Reporter probe | Sg-RP | ATTCTGTCATGCGCTCAC–biotin | 18 |
Linear target | Sg-LT | GACATACCCATTGGTGCAGGTATATGCGCT | 30 |
Mismatched target | Sg-MM | GACATACCCATTGGACCAGGTATATGCGCT | 30 |
