Fig. 2: Resource competition deviates the cell fate transitions in the one-strain Syn-CBS circuit.
From: Winner-takes-all resource competition redirects cascading cell fate transitions
a The normalized steady-state signal intensity of average RFP vs. GFP measured by a plate reader shows a two-phase piecewise linear relationship. Data displayed as mean ± SD (n = 3 biological independent samples). b Flow cytometry data show cell state transitions in one-strain Syn-CBS circuit with increasing level of inducer L-ara (DL-ara). In total, 10,000 events were recorded for each sample. Data shown from one representative of four independent biological replicates. c Diagram of the perturbed state transitions by resource competition. Dash line: expected path. Solid line: perturbed path. d Diagram of the revised model by including resource competition. e Phase plane diagrams. The nullclines of M1 and M2 are shown in green and red, respectively. The vector field of the system is represented by small arrows, where the color is proportional to the field strength. The three cell fates are indicated by filled circles (black, red, and green) at the intersections of the two nullclines. f Calculated potential landscape. Circuit CT61 was used here. Source data are provided as a Source Data file.
