Fig. 2: p53 dynamics vary across tissues.

a Average p53 immunofluorescence intensity in the indicated tissues as a function of time after irradiation (IR, n = 3 samples per tissue, except for the thymus, in which n = 2 at t = 0; error bars are SEM. Dots indicate individual samples). b Representative images from three independent experiments of p53 immunofluorescence at 2 h or 5 h after irradiation. c Representative images and quantification of p53-phosphoS15 immunofluorescence in the small intestine and spleen as a function of time (n = 3 samples per tissue; Error bars are SEM. Dots indicate individual samples). d Quantification of p53 intensity across the crypt of the small intestine in mice treated with IR and analyzed at the indicated time points (n = 125,225,175,275,200,225 cells per time point). e Heat map (blue = low, red = high) of the average p53 intensity from a as a function of time and cellular position within the crypt. f Histograms of p53 immunofluorescence intensity in spleen cells at the indicated time points following irradiation (distributions calculated from >200 cells per time point). g qPCR-based quantification of the fold change (FC) in mRNA levels of p53 target genes MDM2, CDKN1A (p21), and PUMA in the indicated tissues as a function of time. Experiments were done in duplicates.