Fig. 1: Ad-ACE2-transduced Tmprss2-KO mice demonstrates TMPRSS2 as an important factor for SARS-CoV-2 infection. | Nature Communications

Fig. 1: Ad-ACE2-transduced Tmprss2-KO mice demonstrates TMPRSS2 as an important factor for SARS-CoV-2 infection.

From: Distinct mechanisms for TMPRSS2 expression explain organ-specific inhibition of SARS-CoV-2 infection by enzalutamide

Fig. 1: Ad-ACE2-transduced Tmprss2-KO mice demonstrates TMPRSS2 as an important factor for SARS-CoV-2 infection.

a H&E staining of WT and Tmprss2-KO mouse lungs. b YFP IHC staining for lungs, kidneys, livers, and prostates of T2Y mice with (bottom) or without (top) tamoxifen administration. Red arrow indicates alveoli cells and green arrow indicates bronchiole cells, respectively. c Experimental strategy to identify the role of TMPRSS2 in mediating SARS-CoV-2 infection utilizing Ad-ACE2-transduced mouse models. d The percentage of body weight post SARS-CoV-2 infection in initial body weight (two-tailed t-test, mean ± SEM, n = 7 (day 0), n = 7 (day 1), n = 4 (day 2), n = 3 (day 3), n = 3 (day 4), n = 3 (day 5), and n = 3 (day 6) biologically independent mice for wild type group, n = 10 (day 0), n = 10 (day 1), n = 5 (day 2), n = 5 (day 3), n = 5 (day 4), n = 5 (day 5), and n = 5 (day 6) biologically independent mice for Tmprss2-KO group). e Viral loads of SARS-CoV-2 in the lungs of WT and Tmprss2-KO mice (two-tailed t-test, mean ± SEM, n = 5 biologically independent mice). f S protein IHC staining for lungs of Ad-ACE2-transduced wild type mice with (left) or without (right) SARS-CoV-2 challenge and Ad-ACE2-transduced Tmprss2-KO mice (middle) with SARS-CoV-2 challenge, respectively. g Quantification of lung cells with SARS-CoV-2 infection indicated by S protein IHC staining (two-tailed t-test, mean ± SEM, n = 5 biologically independent mice). h H&E staining of lungs from Ad-ACE2-transduced WT mice challenged with (left) or without (right) SARS-CoV-2 and Ad-ACE2-transduced Tmprss2-KO mice (middle) challenged with SARS-CoV-2, respectively. i Immunofluorescence staining for CD45 in the lungs of Ad-ACE2-transduced wild type mice with (left) or without (right) SARS-CoV-2 challenge and Ad-ACE2-transduced Tmprss2-KO mice (middle) with SARS-CoV-2 challenge, respectively. j Quantification for CD45-positive cells in total cells (two-tailed t-test, mean ± SEM, n = 5 biologically independent mice for wild type group and n = 3 biologically independent mice for Tmprss2-KO group). k–n qRT-PCR analyses on mRNA expression of Il6 (k), Cxcl10 (l), Ifnb (m), and Ifng (n) in the lungs of WT and Tmprss2-KO mice challenged by SARS-CoV-2 (two-tailed t-test, mean ± SEM, n = 6 biologically independent mice for wild type group and n = 5 biologically independent mice for Tmprss2-KO group respectively). o H&E score quantification for lung lesions in WT mice and Tmprss2-KO mice (Mann–Whitney test, mean ± SEM, n = 6 biologically independent mice for wild type group and n = 5 biologically independent mice for Tmprss2-KO group). Scale bars represent 50 μm.

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