Fig. 1: Salmonella enterica serovar Typhimurium (STM) infection enhances glycolysis and reduces serine synthesis in infected macrophages. | Nature Communications

Fig. 1: Salmonella enterica serovar Typhimurium (STM) infection enhances glycolysis and reduces serine synthesis in infected macrophages.

From: Salmonella Typhimurium reprograms macrophage metabolism via T3SS effector SopE2 to promote intracellular replication and virulence

Fig. 1

a, b, Extracellular acidification rate (ECAR) (a) and basal oxygen consumption rate (OCR) (b) in untreated (NT) peritoneal macrophages (PMs) or those infected with STM for 2, 8, or 20 h. c, d Fold changes in glucose metabolites (c) and schematic of glucose metabolism (d) in STM-infected (8 h) PMs versus untreated PMs. Metabolites that were significantly enriched (red), diminished (blue), or not significantly changed (green) are indicated (d). glucose 6-phosphate (G6P), fructose 6-phosphate (F6P), fructose-1,6-diphosphate (FBP), glyceraldehyde-3-phosphate (G3P), dihydroxyacetophenone (DHAP), 3-phosphoglycerate (3PG), 2-phosphoglycerate (2PG), phosphoenolpyruvate (PEP), acetyl-CoA (Ac-CoA), citratre (Cit), isocitrate (Ict), α-ketoglutarate(α-KG), succinate (Suc), succinyl coenzyme A (Suc-CoA), fumarate (Fum), malate (Mal), oxaloacetate (OAA), 6-phosphogluconate (6PG), Ribose 5-phosphate (R5P), erythrose 4-phosphate (E4P), xylulose 5-phosphate (X5P), glutathione (GSH). Data are presented as mean ± SD, n = 3 independent experiments (a–c). e Heatmap of the expression profiles of glucose metabolism genes in STM-infected (8 h) PMs versus untreated PMs. Z scores of the relative gene expression levels are displayed in the heatmaps (n = 3 independent experiments), with red representing higher and blue representing lower abundance. P values were determined using one-way ANOVA (a–c, e). *, **, *** P < 0.05, 0.01, 0.001, respectively e. Source data are included in Source Data file.

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