Fig. 2: L1 RNA is modified by m6A.
From: L1 retrotransposons exploit RNA m6A modification as an evolutionary driving force
a MeRIP-qPCR analysis of mRNA from H9 hESCs. Eluates from IgG immunoprecipitation served as negative control. Eluted RNA was quantified to determine the percentage of input. (n = 3 independent samples, mean ± s.e.m., one-way ANOVA and Dunnett’s multiple comparisons test). b MeRIP-qPCR analysis of pL1Hs-transfected HeLa cells with m6A machinery knockdown. Eluted RNAs were quantified using primers specific for reporter L1. The enrichment of RNA was normalized to that of the control. (n = 5 independent samples, mean ± s.e.m., unpaired two-tailed t-test, *p < 0.05). c Map of m6A modification sites in full-length L1Hs from previously reported MeRIP-seq data for H1 hESCs (GSE52600). Read coverage was normalized to the total number of reads mapped to the L1Hs consensus sequence. The plot presents data from MeRIP-seq in red and input RNA-seq in blue. Bars (in red or black) indicate the m6A peaks identified by manual inspection in two replicates. m6A peaks in red correspond to peaks containing high score m6A-prediction sites. Source data are provided as a Source data file.
