Fig. 3: HR-restarted forks are insensitive to the RTS1 barrier.
From: Replication dynamics of recombination-dependent replication forks
a Pu-seq traces for the RTS1-rRFB locus are overlaid for two sets of strains: rtf1+ (WT), which has endogenous rtf1 expression (Pol δ: blue, Pol: ε red) and adh-rtf1+, where expression is under control of the adh1 promoter (Pol δ: black, Pol: ε orange). b Schematic of the tandem RTS1-rRFB locus on chromosome II. The positions of the directional RTS1 and rRFB barriers are shown as red and orange, respectively with the thick bar indicating the directionality of fork arrest. ARS: autonomously replicating sequence. c Pu-seq traces for the tandem RTS1-rRFB locus which contains two copies of the RTS1 barrier that are both orientated to arrest left-right forks (right) compared to the original RTS1-rRFB locus (left). Top: barrier off. Bottom: barrier on.
