Fig. 2: Genome-wide CRISPR/Cas9 screen identifies host factors using Sdel virus as model. | Nature Communications

Fig. 2: Genome-wide CRISPR/Cas9 screen identifies host factors using Sdel virus as model.

From: A genome-wide CRISPR screen identifies host factors that regulate SARS-CoV-2 entry

Fig. 2: Genome-wide CRISPR/Cas9 screen identifies host factors using Sdel virus as model.The alternative text for this image may have been generated using AI.

a Schematic of the screening process. A549 cells expressing the human ACE2 were used to generate the CRISPR sgRNA knockout cell library. The library was infected with Sdel strain of SARS-CoV-2, and cells survived were harvested for genomic extraction and sequence analysis. b Genes and complexes identified from the CRISPR screen. The top 32 (FDR < 0.15) genes were indicated based the MAGeCK score. c The top 32 genes were selected for experimental validation in A549-ACE2 cells using two independent sgRNAs by Sdel live virus infection. Data shown are an average of two independent experiments performed in triplicate and are normalized to the controls of individual experiments. One-way ANOVA with Dunnett’s test; n = 6; mean ± s.d.; ****P < 0.0001.

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