Fig. 2: PD-1Ab21 promotes the differentiation of naïve-like T cells from activated CD8+ T cells. | Nature Communications

Fig. 2: PD-1Ab21 promotes the differentiation of naïve-like T cells from activated CD8+ T cells.

From: Targeting IL-21 to tumor-reactive T cells enhances memory T cell responses and anti-PD-1 antibody therapy

Fig. 2: PD-1Ab21 promotes the differentiation of naïve-like T cells from activated CD8+ T cells.

a, b Flow cytometry analysis of the differentiation of activated OT-1 cells. Lymph node cells from OT-1 mice were primed with OVA257-264 for 2 d. a Three days after differentiation with the indicated proteins, T cell differentiation was analyzed by the expression of CD62L and CD44 on gated live CD8+ T cells (left). Column graphs show the relative frequencies of different cell populations (right). b The activated CD44highCD62Lhigh CD8+ T cells were sorted and differentiated with PD-1Ab21 or medium alone for 3 days. Cells were counted and analyzed by flow cytometry (left). Column graphs show cell numbers of different cell populations (right). c Proliferation of differentiated cells. Activated CD44highCD62Lhigh CD8+ T cells were sorted and labeled with CFSE. Three days after differentiation with PD-1Ab21, cell proliferation was measured by CFSE dilution. Numbers show the percentage of CD44lowCD62Lhigh cells in the dot-plot quadrants (left) or the percentages of cells with more than two divisions (right). All data are representative of at least three independently performed experiments. Results are presented as mean ± SEM, and were compared by unpaired two-tailed t-test. a ****p < 0.0001, b ****p < 0.0001, **p = 0.0033 (d1), **p = 0.0086 (d2), **p = 0.0015 (d3), n.s. not significant.

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