Fig. 7: Argonaute WAGO-1 is required for PGL-1 tethered mRNA reporter repression.
From: C. elegans germ granules require both assembly and localized regulators for mRNA repression
a Protein–mRNA tethering assay and WAGO-1. To test the necessity of WAGO-1 for mRNA repression, PGL-1::SNAP::λN22 germlines were analyzed for GFP reporter expression in the presence or absence of WAGO-1. N-terminal dimerization domain (NtDD, yellow), central dimerization domain (CDD, orange), SNAP (magenta), λN22 (light blue) and RGG repeats (blue), and GFP (green). Modified from76. b–e GFP reporter expression in germ cells of live animals with (b, d) wild-type wago-1 or (c, e) wago-1 null. (b, c) Brightfield image. (d, e) GFP fluorescence (green); auto fluorescence (red). n, number of animals scored for GFP expression. Scale bar, 10 μm, in (b) applies to (b–e) images. f–i Representative images of fixed germlines with (f, h) wild-type wago-1 or (g, i) wago-1 null. (f, g) GFP fluorescence (green). (h, i) DNA (DAPI, cyan) and PGL-1 granule formation seen by SNAP staining (magenta). n, number of germlines scored for GFP expression. Fisher’s exact test of wago-1 wild-type vs. null (p-value < 0.0001). Scale bar, 10 μm, in (f) applies to images (f–i).
