Fig. 1: Steps of DIGAP procedures.
From: Automatic deep learning-driven label-free image-guided patch clamp system
1: Pipette calibration by the user, 2: pipette replacement after recording, 3: image-based automatic pipette tip detection, 4: automatic cell detection, 5: pipette navigation to the target cell, 6: 3D cell tracking, 7: pressure regulation, 8: gigaseal formation, 9: break-in, 10: electrophysiological recording, 11: nucleus and cytoplasm harvesting, 12: anatomical reconstruction of the recorded cell.
