Fig. 5: Monocle single-cell trajectory of tumor-infiltrating CD4+ TC from breast cancer patients. | Nature Communications

Fig. 5: Monocle single-cell trajectory of tumor-infiltrating CD4+ TC from breast cancer patients.

From: Common clonal origin of conventional T cells and induced regulatory T cells in breast cancer patients

Fig. 5

Pseudotime transcriptome analysis of tumor tissue (TT)-derived CD4+ TC from four patients. a, b All TT CD4+ TC are depicted colored by (a) cell-surface phenotype (TT Tconv, green; TT ActTconv, red; TT Treg, blue) or (b) single-cell transcriptome cluster (0: red, 1: orange, 2: green, 3: blue, 4: purple). c, d All expanded (n≥2 single cells) TCRαβ clones (unique paired TCRαβ nucleotide sequences) are depicted colored by (c) cell-surface phenotype (TT Tconv, green; TT ActTconv, red; TT Treg, blue) or (d) transcriptome cluster (0: red, 1: orange, 2: green, 3: blue, 4: purple) on a gray background of unexpanded clones (n = 1 single-cell). Each particular shape corresponds to a different unique TCRαβ clone. TCRαβ clones a-c are shared between tumor-infiltrating Tconv and circulating MAMI-reactive IFNγ+Teff, while clones 1–8 are shared between different (c) cell-surface phenotypes or (d) clusters within the tumor.

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