Fig. 2: Adiposity of Wistar and GK rats.
From: The Goto-Kakizaki rat is a spontaneous prototypical rodent model of polycystic ovary syndrome
a Lee index of 3 and 6 month-old Wistar (W, open symbols) and GK (closed symbols) rats. b Amounts of fat mass and lean mass expressed as a percentage of body weight of 3 month-old Wistar and GK rats. c–f Weight of inguinal (c), perirenal (d), parametrial (e), and periovarian (f) fat depots expressed as the percentage of the body weight in 3 and 6 month-old Wistar and GK rats. g Representative abdominal cavity (left panel) and periovarian and parametrial fat (right panel) of Wistar (n = 8) and GK (n = 9) rats at 3 months. Black arrow: ovary; white arrow: oviduct; black arrowhead: periovarian fat; white arrowhead: parametrial fat. h Assessment of serum leptin levels in 3 and 6 month-old Wistar and GK rats. i Adipocytes size measurement on histological sections (j) from 3 month-old Wistar (dotted line) and GK (full line) rat periovarian adipose tissue, expressed as percentage of adipocytes in each size class. Statistical differences in adipocyte surface distribution between GK and Wistar rats were analyzed by χ2 (p < 0.0001). The inset in (i) shows the mean ± SEM adipocyte surface in Wistar and GK rats. j Representative images of 3 month-old Wistar (n = 6) and GK (n = 5) rats periovarian adipose tissue sections (Scale bar = 100 µm). k Expression of marker genes of triglyceride esterification (Gpat (white), Dgat (peach), lipogenesis (Acaca (salmon), Fasn (green)), adipocyte differentiation (Pparγ (blue)) and lipolysis (Lipe (purple)) in 3 month-old GK rats periovarian adipose tissue, expressed as box with scatter blot with mean ± SEM (n = 5). Unless indicated, data are represented as scatter blot with mean ± SEM. p-values are from two-sided Mann–Whitney U test for unpaired comparisons (GK vs. Wistar rats). n represents the number of biologically independent animals in each group. Source data are provided as a Source Data file.
