Fig. 2: Pf-derived EVs are enriched with kinases and proteasome subunits.
From: 20S proteasomes secreted by the malaria parasite promote its growth
Gene Ontology (GO) cellular components enrichment analysis of Pf-derived EV protein cargo. Vesicles were harvested and proteins were extracted and subjected to cellular components analysis following LC-MS/MS proteomic identification. The horizontal bar graph shows the fold enrichment of significantly enriched A Pf and D human proteins. Results are based on GO cellular component enrichment analysis of all identified proteins against the background of D Homo sapiens and A Plasmodium falciparum (http://geneontology.org/; p-value < 0.05, FDR < 0.01). Lists of identified Pf and human (B and E) kinases and proteasome subunits (C and F). G Phosphoproteomics analysis of uRBCs treated with Pf-derived EVs. Identified proteins were subjected to GO Biological Processes analysis (FDR < 0.00035). Statistical analyses in A and D were done using PANTHER Statistical Overrepresentation Test (Released 2019-07-11). Annotation versions and release date: GO Ontology database (Released 2019-10-08), PANTHER version 15 (Released 2020-02-14). Analyzed lists: Homo sapiens or Plasmodium falciparum Proteins IDs (Uniprot Accessions are listed in Table S1A, S1B). Reference list: Homo sapiens or Plasmodium falciparum (all genes in database). Test Type: FISHER, applying FDR correction, p < 0.05.
