Fig. 3: Programmable assembly of ssDNA-12 in DPNF via HCR of DNA hairpins. | Nature Communications

Fig. 3: Programmable assembly of ssDNA-12 in DPNF via HCR of DNA hairpins.

From: Spatiotemporally programmable cascade hybridization of hairpin DNA in polymeric nanoframework for precise siRNA delivery

Fig. 3: Programmable assembly of ssDNA-12 in DPNF via HCR of DNA hairpins.

A Schematic description of assembling ssDNA-12 in DPNFs: the ssDNA-12 was linked with H2 to form HA first, and then H1 (3 μM) and HA (3 μM) was co-incubated with DPNFs (0.42 mg/ml). B Gel electrophoresis analysis of loading of H1 (3 μM) and HA (3 μM) in DPNF-10 or DPNF-20 (0.42 mg/ml) versus incubation time according to the strategy represented in (A). D, E are quantitative analysis of unloaded HA versus incubation time in (B) and (C), respectively, using image J software. Error bars represent s.d. (n = 3).

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