Fig. 4: PBA mediating cellular uptake and lysosomal escape of DPNF. | Nature Communications

Fig. 4: PBA mediating cellular uptake and lysosomal escape of DPNF.

From: Spatiotemporally programmable cascade hybridization of hairpin DNA in polymeric nanoframework for precise siRNA delivery

Fig. 4: PBA mediating cellular uptake and lysosomal escape of DPNF.

A Schematic illustration of PBA mediating cellular uptake and lysosomal escape of DPNFs. B Flow cytometry analysis of cellular uptake of Cy5-SDPNF-20 (420 μg/ml) by MBA-MD-231 cells at different incubation time. C CLSM images of TAMRA-SDPNF-20 treated MBA-MD-231. BA-block group, cells were pretreated with 4-boronobenzoic acid (BA, 0.2 mg/ml) and then incubated with TAMRA-SDPNF-20 (420 μg/ml); No-block group, cells were incubated with TAMRA-DPNF-20 without BA pretreatment. D CLSM images of MBA-MD-231 cells incubated with Cy5-DPNF-20 (420 μg/ml) at different time points. Cellular nuclei (blue) were stained by 4′,6-diamidino-2-phenylindole (DAPI) and lysosomes were stained with LysoTracker Green.

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