Fig. 2: EBB3 over- and under-expressing lines show early and late bud-break phenotypes respectively.
From: EARLY BUD-BREAK 1 and EARLY BUD-BREAK 3 control resumption of poplar growth after winter dormancy
a Early bud-break of a representative over-expressing line compared to WT-717 control plant. b EBB3 relative expression in EBB3-OE lines. c Time to bud-break in WT-717 plants and EBB3-OE lines. d Delayed bud-break in representative EBB3 under-expressing line compared to WT-T89 control plant. e EBB3 relative expression in EBB3-RNAi lines. f Time to bud-break relative to WT-T89 control plants in EBB3-RNAi lines. Expression values are average of three biological replicates ±SEM, normalized to the reference ACT7 for WT-717 and UBQ gene for WT-T89 clones, respectively. Asterisks (*) indicate significant and (**) indicate extremely significant differences compared to WT at P < 0.05 and P < 0.0001, respectively, and determined by two-tailed paired t-tests. Source data underlying Fig. 2a, c, and d are provided as a Source Data file.
